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Image Search Results
Journal: Oncology Letters
Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer
doi: 10.3892/ol.2018.9265
Figure Lengend Snippet: SRA promoted cell proliferation in SiHa cells. (A) Map of plasmid pLenti6/V5-D-TOPO and the sequence of the overexpression fusion gene SRA. (B) Overexpression of SRA in SiHa cells was analyzed using reverse transcription-quantitative polymerase chain reaction. (C) Cell proliferation was analyzed using Cell Counting kit-8 assays. Bars indicate the mean ± standard deviation of three independent experiments. *P<0.05 vs. SiHa, Vector cells. SD, standard deviation; SRA, steroid receptor activator.
Article Snippet: Cell lines and cell culture The human
Techniques: Plasmid Preparation, Sequencing, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting, Standard Deviation
Journal: Oncology Letters
Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer
doi: 10.3892/ol.2018.9265
Figure Lengend Snippet: SRA promoted cell migration and invasion. (A) Wound healing assay observed under the optical microscope were used to determine migration in SRA-overexpressing SiHa cells (×200). Cells after 24 and 48 h analyzed and determine for SiHa cells as a control. (B) Presents the wound healing assay percentages of each cell line. (C) Cell invasion observed under the optical microscope. Matrigel invasion assays were used to determine invasion after 48 h in SRA-overexpressing SiHa cells. (D) Presents the Matrigel invasion assay percentages of each cell line. Each assay was performed in triplicate. Data are means ± standard deviation. **P<0.01 vs. SiHa, Vector cells. ***P<0.0001 vs. SiHa, Vector cells. SRA, steroid receptor activator.
Article Snippet: Cell lines and cell culture The human
Techniques: Migration, Wound Healing Assay, Microscopy, Control, Invasion Assay, Standard Deviation, Plasmid Preparation
Journal: Oncology Letters
Article Title: Expression levels of the long noncoding RNA steroid receptor activator promote cell proliferation and invasion and predict patient prognosis in human cervical cancer
doi: 10.3892/ol.2018.9265
Figure Lengend Snippet: Effects of overexpression SRA on EMT-associated genes in SiHa cells. (A) Levels of E-cadherin, N-cadherin, Snail, β-catenin, Wnt5β, Vimentin and Twist were analyzed by reverse transcription-quantitative polymerase chain reaction in SRA overexpression SiHa cells. (B) Protein lysates were obtained from SRA-overexpressing SiHa cells. Levels of proteins in EMT-associated gene were analyzed using western blotting. (C) The band intensities were quantitated. The histogram revealed the average volume density corrected for the loading control (β-actin). *P<0.05 vs. SiHa cells. SRA, steroid receptor activator; EMT, epithelial-mesenchymal transition.
Article Snippet: Cell lines and cell culture The human
Techniques: Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control